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tryple select enzyme 1x  (Thermo Fisher)


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    Thermo Fisher tryple select enzyme 1x
    Tryple Select Enzyme 1x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+tryple+select/tryple+express+enzyme/pm40422209-53-16-20
    Average 90 stars, based on 1 article reviews
    tryple select enzyme 1x - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Establishment and Validation of Patient-Derived Non-Small Cell Lung Cancer Organoids as In Vitro Lung Cancer Models
    Article Snippet: .. For splitting, the supernatant was removed and the Matrigel drop was resuspended in 1 mL 1X TrypLE select (ThermoFisher) and incubated at 37 ◦C for 5–10 min under close surveillance for organoid dissociation. ..

    Article Title: Establishment and Validation of Patient-Derived Non-Small Cell Lung Cancer Organoids as In Vitro Lung Cancer Models
    Article Snippet: .. For splitting, the supernatant was removed and the Matrigel drop was resuspended in 1 mL 1X TrypLE select (ThermoFisher) and incubated at 37 °C for 5–10 min under close surveillance for organoid dissociation. ..

    Saline:

    Article Title: Effect of a FOXO1 inhibitor on trophoblast differentiation from human pluripotent stem cells and ERV-associated gene expression
    Article Snippet: The validated human iPSC line 201B7 was obtained from the RIKEN Cell Bank (Tsukuba, Japan) and transferred from on-feeder to feeder-free conditions in our laboratory. iPSCs were maintained in StemFit AK02 N medium (Ajinomoto, Tokyo, Japan) supplemented with penicillin (50 units/mL) and streptomycin (50 μg/mL) (Gibco, Carlsbad, CA, USA) at 37 °C and 5% CO 2 . .. For passages (every 7 days), iPSCs were dissociated into single cells by treatment with 0.5X TrypLE Select (1X TrypLE Select [Thermo Fisher Scientific, Waltham, MA, USA] diluted 1:1 with 0.5 mM EDTA [Nacalai Tesque, Kyoto, Japan] in phosphate-buffered saline [PBS]) and seeded with StemFit medium with 10 μM Y-27632 (034–24024; Wako, Osaka, Japan) and iMatrix-511 silk (0.167 μg/cm 2 ; Nippi, Tokyo, Japan) in uncoated usage. ..

    Flow Cytometry:

    Article Title: Hypoimmunogenic hPSC-derived cardiac organoids for immune evasion and heart repair
    Article Snippet: .. Cells were prepared for flow cytometry via dissociation with 1X TrypLE Select for differentiated cells (Thermo Fisher Scientific) or Accutase for hPSCs. ..

    other:

    Article Title: Modeling the atrioventricular conduction axis using human pluripotent stem cell-derived cardiac assembloids
    Article Snippet: 1x TryPLE Select , ThermoFisher Scientific , Cat# 12563011.

    Control:

    Article Title: Modeling the atrioventricular conduction axis using human pluripotent stem cell-derived cardiac assembloids
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Cardiac Troponin T Antibody, anti-human/ mouse/rat, APC, REAfinity Miltenyi Biotec Cat#130-120-403; RRID:AB_2783887 REA isotype Control-APC, human IgG1, REAfinity Miltenyi Biotec Cat#130-120-709; RRID:AB_2784399 Mouse Monoclonal anti-a-Actinin Antibody Sigma-Aldrich Cat# A7811; RRID:AB_476766 Rabbit Polyclonal anti-T-box 2 Antibody Peprotech Cat# 16930-1-AP; RRID:AB_2200526 Rabbit Polyclonal Anti-Homeobox protein MSX-2 Antibody Millipore Cat# HPA005652; RRID:AB_1079418 Rabbit Polyclonal anti-Myosin Light Chain 2 Antibody Proteintech Group Cat#10906-1-AP; RRID:AB_2147453 Mouse Monoclonal anti-Human COUP-TF II/NR2F2 Antibody R&D systems Cat# PP-H7147-00; RRID:AB_2155627 Rabbit Polyclonal anti-Cardiac Troponin T Antibody Abcam Cat# Ab45932; RRID:AB_956386 Donkey anti-rabbit Alexa Fluor 647 Antibody Invitrogen Cat# A-31573; RRID:AB_2536183 Donkey anti-Mouse Alexa Fluor 647 Antibody Invitrogen Cat# A-31571; RRID:AB_162542 Donkey anti-Rabbit Alexa Fluor 488 Antibody Invitrogen Cat# A-21206; RRID:AB_2535792 Donkey anti-Mouse Alexa Fluor 488 Antibody Invitrogen Cat# A-21202; RRID:AB_141607 Chemicals, peptides, and recombinant proteins Di-4 ANNEPS ANASPEC INC. Cat# AS-84723 Blebbistatin Cayman Chemical Cat# 13013 Sodium Chloride (NaCl) Millipore Cat# 567440-1KG Potassium Chloride (KCl) Sigma-Aldrich Cat# 104936 Calcium Chloride (CaCl2) Sigma-Aldrich Cat# 102378-500G Magneisum Chloride Hexahydrate (MgCl2*6H2O) Sigma-Aldrich Cat# 105833 D(+)-Glucose Monohydrate Millipore Cat# 108342 HEPES Sigma-Aldrich Cat# H3375 Sodium Hydroxide (NaOH) Sigma-Aldrich Cat# S5881 K-gluconate Sigma-Aldrich Cat# G4500 Amphotericin-B Sigma-Aldrich Cat# A4888-250MG DMSO Sigma-Aldrich Cat# D2650 Ivabradine Tocris Cat# 6542 Carbamoylcholine chloride (Carbachol) Sigma-Aldrich Cat# C4382-1G Indo-1 AAT-Bioquest Cat# 21030 Caffeine Sigma-Aldrich Cat# C0750-5G Nickel Chloride (NiCl2) Sigma-Aldrich Cat# 806722 1x TryPLE Select ThermoFisher Scientific Cat# 12563011 Fibronectin Corning Cat# 356008 BIO-AMF2 medium Biological Industries Cat# 01-194-1 Growth Factor Reduced Matrigel, PhenolRed free Corning Cat# 356231 (Continued on next page) Cell Stem Cell 31, 1667–1684.e1–e6, November 7, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Growth Factor Reduced Matrigel Corning Cat# 354230 mTESR1 medium Stem Cell Technologies Cat# 5850 EDTA Invitrogen Cat# 15575-038 Activin-A Miltenyi Biotec Cat# 3130-115-012 BMP4 R&D systems Cat# 314-BP-101/CF CHIR99021 Axon Medchem Cat# 1386 XAV939 Bioscience Cat# 3748/10 RA Sigma-Aldrich Cat# R2625-50MG PD173074 Selleck Chemicals Cat# 1264 BMS753 Tocris Cat# 3505 WNT2 Sigma-Aldrich Cat# SRP6560-10UG SB431542 Tocris Cat# 1614 Fixation Buffer Biolegend Cat# 420801 Intracellular staining buffer Biolegend Cat# 421002 dNTP set (100nM) Solis Biodyne Cat# 02-21-00100 LightCycler 480 SYBR Green I Master Roche Cat# 4887352001 Paraformaldehyde VWR Cat# 28794.295 Triton-X Sigma-Aldrich Cat# T8787 normal swine serum Jackson Immunoresearch Cat# 014-000-121 DAPI Sigma-Aldrich Cat# D9542 Critical commercial assays CytoTune 2.0 Sendai Reprogramming Kit ThermoFisher Scientific Cat# A16517 Human Stem Cell Nucleofector Kit Lonza Cat# VPH-5012 Nucleospin RNA kit Machery Nagel Cat# MN740955.50 Superscript II ThermoFisher Scientific Cat# 18064071 Deposited data scRNA-seq data: hiPSC-derived W+R cardiomyocytes (AVCM) (Figure 1E) This Manuscript NCBI GEO: GSE250173 scRNA-seq data; hiPSC-derived SANCM, ACM and VCM (Figure 1C) Wiesinger et al.18 and Li et al.19 NCBI GEO: GSE189782 scRNA-seq data; hiPSC-derived AVC cardioids Schmidt et al.12 NCBI GEO: GSE239891 scRNA-seq data; hESC-derived AVC cardiomyocytes Ye et al.13 NCBI GEO: GSE238107 scRNA-seq data; Mouse Heart de Soysa et al.21 NCBI GEO: GSE126128 scRNA-seq data; Mouse Heart Hill et al.22 NCBI GEO: GSE131181 Script for scRNA-seq analysis This Manuscript ZENODO: https://doi.org/10.5281/zenodo.

    Recombinant:

    Article Title: Modeling the atrioventricular conduction axis using human pluripotent stem cell-derived cardiac assembloids
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Cardiac Troponin T Antibody, anti-human/ mouse/rat, APC, REAfinity Miltenyi Biotec Cat#130-120-403; RRID:AB_2783887 REA isotype Control-APC, human IgG1, REAfinity Miltenyi Biotec Cat#130-120-709; RRID:AB_2784399 Mouse Monoclonal anti-a-Actinin Antibody Sigma-Aldrich Cat# A7811; RRID:AB_476766 Rabbit Polyclonal anti-T-box 2 Antibody Peprotech Cat# 16930-1-AP; RRID:AB_2200526 Rabbit Polyclonal Anti-Homeobox protein MSX-2 Antibody Millipore Cat# HPA005652; RRID:AB_1079418 Rabbit Polyclonal anti-Myosin Light Chain 2 Antibody Proteintech Group Cat#10906-1-AP; RRID:AB_2147453 Mouse Monoclonal anti-Human COUP-TF II/NR2F2 Antibody R&D systems Cat# PP-H7147-00; RRID:AB_2155627 Rabbit Polyclonal anti-Cardiac Troponin T Antibody Abcam Cat# Ab45932; RRID:AB_956386 Donkey anti-rabbit Alexa Fluor 647 Antibody Invitrogen Cat# A-31573; RRID:AB_2536183 Donkey anti-Mouse Alexa Fluor 647 Antibody Invitrogen Cat# A-31571; RRID:AB_162542 Donkey anti-Rabbit Alexa Fluor 488 Antibody Invitrogen Cat# A-21206; RRID:AB_2535792 Donkey anti-Mouse Alexa Fluor 488 Antibody Invitrogen Cat# A-21202; RRID:AB_141607 Chemicals, peptides, and recombinant proteins Di-4 ANNEPS ANASPEC INC. Cat# AS-84723 Blebbistatin Cayman Chemical Cat# 13013 Sodium Chloride (NaCl) Millipore Cat# 567440-1KG Potassium Chloride (KCl) Sigma-Aldrich Cat# 104936 Calcium Chloride (CaCl2) Sigma-Aldrich Cat# 102378-500G Magneisum Chloride Hexahydrate (MgCl2*6H2O) Sigma-Aldrich Cat# 105833 D(+)-Glucose Monohydrate Millipore Cat# 108342 HEPES Sigma-Aldrich Cat# H3375 Sodium Hydroxide (NaOH) Sigma-Aldrich Cat# S5881 K-gluconate Sigma-Aldrich Cat# G4500 Amphotericin-B Sigma-Aldrich Cat# A4888-250MG DMSO Sigma-Aldrich Cat# D2650 Ivabradine Tocris Cat# 6542 Carbamoylcholine chloride (Carbachol) Sigma-Aldrich Cat# C4382-1G Indo-1 AAT-Bioquest Cat# 21030 Caffeine Sigma-Aldrich Cat# C0750-5G Nickel Chloride (NiCl2) Sigma-Aldrich Cat# 806722 1x TryPLE Select ThermoFisher Scientific Cat# 12563011 Fibronectin Corning Cat# 356008 BIO-AMF2 medium Biological Industries Cat# 01-194-1 Growth Factor Reduced Matrigel, PhenolRed free Corning Cat# 356231 (Continued on next page) Cell Stem Cell 31, 1667–1684.e1–e6, November 7, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Growth Factor Reduced Matrigel Corning Cat# 354230 mTESR1 medium Stem Cell Technologies Cat# 5850 EDTA Invitrogen Cat# 15575-038 Activin-A Miltenyi Biotec Cat# 3130-115-012 BMP4 R&D systems Cat# 314-BP-101/CF CHIR99021 Axon Medchem Cat# 1386 XAV939 Bioscience Cat# 3748/10 RA Sigma-Aldrich Cat# R2625-50MG PD173074 Selleck Chemicals Cat# 1264 BMS753 Tocris Cat# 3505 WNT2 Sigma-Aldrich Cat# SRP6560-10UG SB431542 Tocris Cat# 1614 Fixation Buffer Biolegend Cat# 420801 Intracellular staining buffer Biolegend Cat# 421002 dNTP set (100nM) Solis Biodyne Cat# 02-21-00100 LightCycler 480 SYBR Green I Master Roche Cat# 4887352001 Paraformaldehyde VWR Cat# 28794.295 Triton-X Sigma-Aldrich Cat# T8787 normal swine serum Jackson Immunoresearch Cat# 014-000-121 DAPI Sigma-Aldrich Cat# D9542 Critical commercial assays CytoTune 2.0 Sendai Reprogramming Kit ThermoFisher Scientific Cat# A16517 Human Stem Cell Nucleofector Kit Lonza Cat# VPH-5012 Nucleospin RNA kit Machery Nagel Cat# MN740955.50 Superscript II ThermoFisher Scientific Cat# 18064071 Deposited data scRNA-seq data: hiPSC-derived W+R cardiomyocytes (AVCM) (Figure 1E) This Manuscript NCBI GEO: GSE250173 scRNA-seq data; hiPSC-derived SANCM, ACM and VCM (Figure 1C) Wiesinger et al.18 and Li et al.19 NCBI GEO: GSE189782 scRNA-seq data; hiPSC-derived AVC cardioids Schmidt et al.12 NCBI GEO: GSE239891 scRNA-seq data; hESC-derived AVC cardiomyocytes Ye et al.13 NCBI GEO: GSE238107 scRNA-seq data; Mouse Heart de Soysa et al.21 NCBI GEO: GSE126128 scRNA-seq data; Mouse Heart Hill et al.22 NCBI GEO: GSE131181 Script for scRNA-seq analysis This Manuscript ZENODO: https://doi.org/10.5281/zenodo.



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